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De Duve C Tissue fractionation. Journal of Cell Biology Journal of Biomolecular Techniques Reid E ed Methodological Developments in Biochemistry, vol.

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Analytica Chimica Acta Schumaker VN Zone centrifugation. Advances in Biological and Medical Physics Technical Information, Beckman Coulter. Abstract Centrifuges are used in cell and molecular biology departments, in industrial laboratory settings and medical laboratories.

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Flow chart summarising the sequential steps in the subfractionation of a homogenate by differential centrifugation. The density of a gradient may be increased continuously or discontinuously from top to bottom of the centrifuge tube.

Modes of density gradient centrifugation: Photograph of an actual tissue separation: Buoyancy B , friction F and centrifugal C forces acting on a particle during centrifugation. References Anderson NG Zonal centrifuges and other separation systems. De Duve C Exploring cells with a centrifuge.

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To determine specific activity of enzyme Acid

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